Introduction. The Ecuadorian shrimp industry represents a strategic economic sector and the main non-oil export. Despite international recognition for quality and traceability, deficiencies in cold storage operations compromise efficiency and export standards. The lack of automated temperature control, manual processes, and absence of monitoring systems affect product safety and freshness, leading to economic losses and reduced customer satisfaction. This study aims to optimize the department through technological, operational, and quality control improvements. Objective: To optimize the performance of the cold storage department in shrimp export through technology, process standardization, and human capital development. Specific objectives were to increase loading plan compliance, reduce preparation time, maintain optimal temperature range, minimize product loss, and improve customer satisfaction. Methodology: A mixed-method approach was used, combining quantitative measures of five key indicators pre- and post-intervention, with qualitative data from semi-structured interviews, direct observation, and document review. The population included all cold storage staff and six months of historical records; a non-probabilistic sample of 25 employees was selected. Data was collected using temperature logs, loading control forms, satisfaction surveys, and observation logs. The intervention included IoT sensor installation, transcritical CO₂ refrigeration, standardized processes, and staff training. Data analysis included descriptive and pre–post comparative statistics. Results: Post-intervention, loading plan compliance increased from 16.6% to 109.17%, preparation time decreased from 120 to 30 minutes, temperature range stabilized at 100%, product loss decreased from 6.4% to 0.8%, and customer satisfaction increased from 71% to 94%. The results demonstrate that combining advanced technology, standardized procedures, and continuous training improves operational efficiency, reduces economic losses, and ensures exportable shrimp quality. Conclusion: Integrating IoT monitoring, advanced refrigeration, and staff training optimizes the cold storage department’s performance, ensuring quality, traceability, and international competitiveness of Ecuadorian shrimp. This model is replicable and sustainable for other agro-industrial companies. General Area of Study: Logistics and Supply Chain. Specific area of study: Operations and Process Management. Type of study: Original articles.