Imagin evaluation of platelet-rich fibrin in post-exodontic bone regeneration. A systematic review
Main Article Content
Abstract
Introduction: Tooth extraction is one of the most frequently performed procedures in clinical dentistry. However, it often leads to progressive alveolar ridge resorption, which can compromise future restorative and prosthetic treatments. Objective: This systematic review aimed to examine the role of platelet-rich fibrin (PRF) in post-extraction alveolar ridge preservation, evaluating its effectiveness in enhancing bone tissue regeneration based on radiographic assessments, and comparing its outcomes to those observed with physiological bone healing. Methodology: The review followed PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. A comprehensive literature search was conducted using electronic databases such as PubMed, Scopus, Web of Science, and ScienceDirect. Additionally, gray literature was sourced from the New York Academy of Medicine Grey Literature Report. Only randomized controlled trials (RCTs) were included, and the risk of bias was evaluated using the Cochrane Handbook for Systematic Reviews of Interventions version 6.2 (RevMan 6.2). Results: Seventeen RCTs published up to 2022 met the eligibility criteria, each investigating PRF’s efficacy in bone regeneration following tooth extraction. Conclusion: Although not all results demonstrated statistical significance, the clinical evidence suggests that PRF supports bone neoformation and reduces alveolar bone loss during the healing period, particularly within the first three to four months post-extraction. General field of study: Health Specific field of study: Dentistry Type of study: Systematic review.
Downloads
Download data is not yet available.
Article Details
How to Cite
Rubio Uzho, K. A., Molina Barahona, R. M., Cruz Proaño, E. D., & Vásquez Palacios, A. C. (2025). Imagin evaluation of platelet-rich fibrin in post-exodontic bone regeneration. A systematic review. Anatomía Digital, 8(3.2), 6-28. https://doi.org/10.33262/anatomiadigital.v8i3.2.3458
Section
Articulos de revisión bibliográfica
References
1. Franco Hiza Y, Cortez Velásquez A. Tratamiento del alveolo post-exodoncia. Odontología [Internet]. 2021 [citado 24 mayo 2025]; 6(7):9–14. Disponible en: https://dicyt.uajms.edu.bo/revistas/index.php/odontologia/article/view/969
2. Daugela P, Grimuta V, Sakavicius D, Jonaitis J, Juodzbalys G. Influence of leukocyte- and platelet-rich fibrin (L-PRF) on the outcomes of impacted mandibular third molar removal surgery: A split-mouth randomized clinical trial. Quintessence International [Online]. 2018 [cited 2025 May 24]; 49(5):377–88. Available from: https://pubmed.ncbi.nlm.nih.gov/29629438/
3. Ponte JS, Nogueira AT da F, Maciel JAC, Castro-Silva II. Guided bone regeneration in the anterior maxilla after tooth extraction and implant removal with sticky bone and L-PRF membrane in two surgical approaches. Research, Society and Development [Online]. 2021 [cited 2025 May 24];10(11): e112101119456. Available from: https://rsdjournal.org/index.php/rsd/article/view/19456
4. Arce González MA, Díaz Suárez AM, Díaz Hernández M, Hernández Moreno VJ. Fibrina rica en plaquetas y leucocitos: biomaterial autólogo excelente para la regeneración tisular. Medicentro Electrónica [Internet]. 2018 [citado 24 mayo 2025];22(1):19–26. Available from: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1029-30432018000100003
5. León Batallas J, Unda Jaramillo P, Ortiz Onofre A. Caso Clínico: Aplicación de fibrina rica en factores de crecimiento en el tratamiento de osteonecrosis de los maxilares por Bisfosfonatos. OdontoInvestigación [Internet]. 2018 [citado 24 mayo 2025];4(2). Disponible en: https://revistas.usfq.edu.ec/index.php/odontoinvestigacion/article/view/1279
6. Park G, Benalcázar Jalkh EB, Boczar D, Bergamo E-T, Kim H, Kurgansky G, et al. Bone regeneration at extraction sockets filled with leukocyte-platelet-rich fibrin: An experimental pre-clinical study. Medicina oral, patología oral y cirugía bucal [Online]. 2022 [cited 2025 May 24];27(5): e468–e475. Available from: http://dx.doi.org/10.4317/medoral.25462
7. Valenzuela Mencía J, Gutiérrez Corrales A, Hernández A. Efectos de la fibrina rica en plaquetas y leucocitos (L-PRF) en cirugía bucal. Gaceta Dental [Internet]. 2022 [citado 24 mayo 2025]; (342):40–51. Disponible en: https://dialnet.unirioja.es/servlet/articulo?codigo=8225236
8. Murat Afat İ, Tuna Akdoğan E, Gönül O. Effects of leukocyte- and platelet-rich fibrin alone and combined with hyaluronic acid on pain, edema, and trismus after surgical extraction of impacted mandibular third molars. Journal of oral and maxillofacial surgery [Online]. 2018 [cited 2025 May 24]; 76(5):926–932. Available from: https://pubmed.ncbi.nlm.nih.gov/29304325/
9. Castro Rodrigues CMD, Sol I, De Almeida VLL, Jordao Silva C. Application of L-PRF in the field of dental surgery: A brief review. International Journal of Medical Reviews [Online]. 2021 [cited 2025 May 24];8(2):70–73. Available from: http://www.ijmedrev.com/article_133305.html
10. Serafini G, Lollobrigida M, Fortunato L, Mazzucchi G, Lamazza L, Di Nardo D, et al. Postextractive alveolar ridge preservation using L-PRF: Clinical and histological evaluation. Case reports in dentistry [Online]. 2020 [cited 2025 May 24]; 2020:5073519. Available from: https://pubmed.ncbi.nlm.nih.gov/32577315/
11. Fernandes Pacheco R, Neto Custódio AL, Souza DM de, Oliveira Pacheco CL de, Albergaria-Barbosa JR de. Concentrados autólogos de plaquetas y su aplicabilidad en Odontología. Research, Society and Development [Internet]. 2022 [citado 24 may 2025]; 11(15):e501111536838. Available from: https://rsdjournal.org/index.php/rsd/article/download/36838/31231/412612
12. Vallejo Mera D, Aguilera Molina N, Vallejo Mera V, Villacreses Rodríguez A. Efecto de la fibrina rica en plaquetas leucocitarias como biomaterial post-exodoncia para la regeneración tisular en el paciente diabético controlado. Revista Científica Especialidades Odontológicas [Internet]. 2020 [citado 24 mayo 2025]; 3(1):107–115. Available from: https://dialnet.unirioja.es/servlet/articulo?codigo=8266788
13. Canellas JVDS, da Costa RC, Breves RC, de Oliveira GP, Figueredo CM da S, Fischer RG, et al. Tomographic and histomorphometric evaluation of socket healing after tooth extraction using leukocyte- and platelet-rich fibrin: A randomized, single-blind, controlled clinical trial. Journal of cranio-maxillo-facial surgery [Online]. 2020 [cited 2025 May 24]; 48(1):24–32. Available from: https://pubmed.ncbi.nlm.nih.gov/31810848/
14. Sciaini V, Rodríguez K, Bisonni M, Nuñez M, Gomes I, Suárez JM. Utilización de fibrina rica en plaquetas y leucocitos L–PRF en defectos de lesiones periapicales y periodontales de larga evolución. RCOE: Revista del Ilustre Consejo General de Colegios de Odontólogos y Estomatólogos de España [Internet]. 2020 [citado 24 mayo 2025]; 25(2):177–185. Disponible en: https://dialnet.unirioja.es/servlet/articulo?codigo=7447507
15. Farías-Medina M, Cádiz-Marín S, Fonseca-Escobar D, Parada-Fernández F, Montero-Riffo S. Evaluación comparativa de la inflamación de tejidos blandos en pacientes sometidos a exodoncias de terceros molares con y sin uso de PRF. International Journal of Odontostomatology [Internet]. 2021 [citado 24 mayo 2025]; 15(3):674–680. Disponible en: https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300674
16. Greco T, Cabrera P, Bogarín C, Mendoza CI. Exodoncia de tercer molar con posterior utilización de plug y membrana de fibrina rica en plaquetas y leucocitos (L-PRF). Revisión Científica Odontológica UAA [Internet]. 2020 [citado 24 mayo 2025]; 2(1): 12-16. Disponible en: http://revistacientifica.uaa.edu.py/index.php/ReCO-UAA/article/download/915/831
17. Salgado-Peralvo ÁO, Salgado-García Á, Arriba-Fuente L. Nuevas tendencias en regeneración tisular: fibrina rica en plaquetas y leucocitos. Revista Española de Cirugía Oral y Maxilofacial [Internet]. 2017 [citado 24 mayo 2025]; 39(2):91–98. Disponible en: https://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-05582017000200091
18. Marchiolli CL, Lopez Moreno JM, Denardi RJ, Denardi CM, Araujo Recalde JLB, Assunção WG, et al. Platelet- and leukocyte-rich fibrin (L-PRF) in guided bone regeneration procedures. Research, Society and Development [Online]. 2021 [cited 2025 May 24]; 10(12): e130101219113. Available from: https://rsdjournal.org/index.php/rsd/article/download/19113/18257/248773
19. Araújo Júnior MA de, Castro MCSR de, Silva LSM da, Silva LS da, Corrêa CS, Nahmias HLM, et al. Aplicación terapéutica de fibrina rica en plaquetas (L-PRF) en paciente sometido a tercer exodoncia molar. Research, Society and Development [Internet]. 2022 [citado 24 mayo 2025]; 11(1):e21711124853. Disponible en: https://rsdjournal.org/index.php/rsd/article/download/24853/21808/292819
20. ElAmrousy W, Issa DR. Effect of an “autogenous leukocyte platelet-rich fibrin tooth graft” combination around immediately placed implants in periodontally compromised sites: A randomized clinical trial. International journal of dentistry [Online]. 2022 [cited 2025 May 24]; 2022:4951455. Available from: https://pubmed.ncbi.nlm.nih.gov/35242191/
21. Castro AB, Meschi N, Temmerman A, Pinto N, Lambrechts P, Teughels W, et al. Regenerative potential of leucocyte- and platelet-rich fibrin. Part B: sinus floor elevation, alveolar ridge preservation and implant therapy. A systematic review. Journal of clinical periodontology [Online]. 2017 [cited 2025 May 24]; 44(2):225–234. Available from: https://pubmed.ncbi.nlm.nih.gov/27891638/
22. Baslarli O, Tumer C, Ugur O, Vatankulu B. Evaluation of osteoblastic activity in extraction sockets treated with platelet-rich fibrin. Medicina oral, patologia oral y cirugia bucal [Online]. 2015 [cited 2025 May 24]; 20(1): e111–e116. Available from: https://pubmed.ncbi.nlm.nih.gov/25475771/
23. Lee C-H, Tsai C-Y, Li C-C, Chien C-C, Liu J-W. Teicoplanin therapy for MRSA bacteremia: a retrospective study emphasizing the importance of maintenance dosing in improving clinical outcomes. The Journal of Antimicrobial Chemotherapy [Online]. 2015 [cited 2025 May 24]; 70(1):257–263. Available from: https://pubmed.ncbi.nlm.nih.gov/25190719/
24. Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. Declaración Prisma 2020: Una guía actualizada para la publicación de revisiones sistemáticas. Revista Española de Cardiología [Internet]. 2021 [cited 2025 May 24];74(9):790–799. Disponible en: https://www.revespcardiol.org/es-declaracion-prisma-2020-una-guia-articulo-S0300893221002748
25. Da Costa CM, da Mattos Pimenta CA, Nobre MR. The PICO strategy for the research question construction and evidence search. Revista Latino-Americana de Enfermagem [Online]. 2007 [cited 2025 May 24]; 15(3): 508-511. Available from: https://pubmed.ncbi.nlm.nih.gov/17653438/
26. Higgins JPT, Green, S. Manual Cochrane de Revisiones Sistemáticas de Intervenciones. The Cochrane Collaboration. https://es.cochrane.org/sites/es.cochrane.org/files/uploads/Manual_Cochrane_510_reduit.pdf
27. Boora P, Rathee M, Bhoria M. Effect of Platelet Rich Fibrin (PRF) on peri-implant soft tissue and crestal bone in one-stage implant placement: a randomized controlled trial. Journal of Clinical and Diagnostic Research [Online]. 2015 [cited 2025 May 24];9(4): ZC18–ZC21. Available from: https://pubmed.ncbi.nlm.nih.gov/26023636/
28. Chappuis V, Engel O, Reyes M, Shahim K, Nolte L-P, Buser D. Ridge alterations post-extraction in the esthetic zone: a 3D analysis with CBCT. Journal of Dental Research [Online]. 2013 [cited 2025 May 24]; 92(12 Suppl):195S–201S. Available from: https://pubmed.ncbi.nlm.nih.gov/24158340/
29. Dohan Ehrenfest DM, Andia I, Zumstein MA, Zhang C-Q, Pinto NR, Bielecki T. Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications, and perspectives. Muscles, Ligaments and Tendons Journal [Online]. 2014 [cited 2025 May 24]; 4(1):3–9. Available from: https://pubmed.ncbi.nlm.nih.gov/24932440/
30. Farmer M, Darby I. Ridge dimensional changes following single-tooth extraction in the aesthetic zone. Clinical oral implants research [Online]. 2014 [cited 2025 May 24]; 25(2): 272–277. Available from: https://pubmed.ncbi.nlm.nih.gov/23346895/
31. Hamzacebi B, Oduncuoglu B, Alaaddinoglu EE. Treatment of peri-implant bone defects with platelet-rich fibrin. The International Journal of Periodontics & Restorative Dentistry [Online]. 2015 [cited 2025 May 24]; 35(3):415–422. Available from: https://pubmed.ncbi.nlm.nih.gov/25909530/
32. Strauss FJ, Stähli A, Gruber R. The use of platelet-rich fibrin to enhance the outcomes of implant therapy: a systematic review. Clinical Oral Implants Research [Online]. 2018 [cited 2025 May 24]; 29 Suppl 18(Suppl 18):6–19. Available from: https://pubmed.ncbi.nlm.nih.gov/30306698/
33. Nizam N, Eren G, Akcalı A, Donos N. Maxillary sinus augmentation with leukocyte and platelet-rich fibrin and deproteinized bovine bone mineral: a split-mouth histological and histomorphometric study. Clinical Oral Implants Research [Online]. 2018 [cited 2025 May 24]; 29(1):67–75. Available from: https://pubmed.ncbi.nlm.nih.gov/28786494/
34. Kanayama T, Horii K, Senga Y, Shibuya Y. Crestal approach to sinus floor elevation for atrophic maxilla using platelet-rich fibrin as the only grafting material: a 1-year prospective study. Implant Dentistry [Online]. 2016 [cited 2025 May 24]; 25(1): 32–38. Available from: https://pubmed.ncbi.nlm.nih.gov/26384097/
35. Khorshidi H, Raoofi S, Bagheri R, Banihashemi H. Comparison of the mechanical properties of early leukocyte- and platelet-rich fibrin versus PRGF/Endoret membranes. International Journal of Dentistry [Online]. 2016 [cited 2025 May 24]; 2016: 1849207. Available from: https://pubmed.ncbi.nlm.nih.gov/26880919/
36. Marenzi G, Riccitiello F, Tia M, di Lauro A, Sammartino G. Influence of leukocyte- and platelet-rich fibrin (L-PRF) in the healing of simple postextraction sockets: a split-mouth study. Biomed Research International [Online]. 2015 [cited 2025 May 24]; 2015:369273. Available from: https://pubmed.ncbi.nlm.nih.gov/26273612/
37. Öncü E, Alaaddinoğlu EE. The effect of platelet-rich fibrin on implant stability. The International Journal of Oral & Maxillofacial Implants [Online]. 2015 [cited 2025 May 24]; 30(3):578–582. Available from: https://pubmed.ncbi.nlm.nih.gov/26009908/
38. Girish Rao S, Bhat P, Nagesh KS, Rao GHR, Mirle B, Kharbhari L, et al. Bone regeneration in extraction sockets with autologous platelet rich fibrin gel. Journal of Maxillofacial and Oral Surgery [Online]. 2013 [cited 2025 May 24];12(1):11–16. Available from: https://pubmed.ncbi.nlm.nih.gov/24431807/
39. Singh A, Kohli M, Gupta N. Platelet rich fibrin: a novel approach for osseous regeneration. Journal of Maxillofacial and Oral Surgery [Online]. 2012 [cited 2025 May 24]; 11(4):430–434. Available from: https://pubmed.ncbi.nlm.nih.gov/24293936/
40. Hauser F, Gaydarov N, Badoud I, Vazquez L, Bernard J-P, Ammann P. Clinical and histological evaluation of postextraction platelet-rich fibrin socket filling: a prospective randomized controlled study. Implant Dentistry [Online]. 2013 [cited 2025 May 24];22(3):295–303. Available from: https://pubmed.ncbi.nlm.nih.gov/23644909/
41. Lollobrigida M, Maritato M, Bozzuto G, Formisano G, Molinari A, De Biase A. Biomimetic implant surface functionalization with liquid L-PRF products: in vitro study. BioMed Research International [Online]. 2018 [cited 2025 May 24]; 2018:9031435. Available from: https://pubmed.ncbi.nlm.nih.gov/29854805/
42. Kumar N, Prasad K, Ramanujam L, Ranganath K, Dexith J, Chauhan A. Evaluation of treatment outcome after impacted mandibular third molar surgery with the use of autologous platelet-rich fibrin: a randomized controlled clinical study. Journal of Oral and Maxillofacial Surgery [Online]. 2015 [cited 2025 May 24];73(6):1042–1049. Available from: https://pubmed.ncbi.nlm.nih.gov/25659357/
43. Sameera S, Nagasri M, Aravind Kumar P, Indeevar P, Raviraj K, Musalaiah SVVS. Comparison of two surgical techniques in the treatment of multiple gingival recessions sandwiched with a combination of A-PRF and L-PRF. The Saudi Dental Journal [Online]. 2018 [cited 2025 May 24]; 30(3):183–189. Available from: https://pubmed.ncbi.nlm.nih.gov/29942101/
2. Daugela P, Grimuta V, Sakavicius D, Jonaitis J, Juodzbalys G. Influence of leukocyte- and platelet-rich fibrin (L-PRF) on the outcomes of impacted mandibular third molar removal surgery: A split-mouth randomized clinical trial. Quintessence International [Online]. 2018 [cited 2025 May 24]; 49(5):377–88. Available from: https://pubmed.ncbi.nlm.nih.gov/29629438/
3. Ponte JS, Nogueira AT da F, Maciel JAC, Castro-Silva II. Guided bone regeneration in the anterior maxilla after tooth extraction and implant removal with sticky bone and L-PRF membrane in two surgical approaches. Research, Society and Development [Online]. 2021 [cited 2025 May 24];10(11): e112101119456. Available from: https://rsdjournal.org/index.php/rsd/article/view/19456
4. Arce González MA, Díaz Suárez AM, Díaz Hernández M, Hernández Moreno VJ. Fibrina rica en plaquetas y leucocitos: biomaterial autólogo excelente para la regeneración tisular. Medicentro Electrónica [Internet]. 2018 [citado 24 mayo 2025];22(1):19–26. Available from: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1029-30432018000100003
5. León Batallas J, Unda Jaramillo P, Ortiz Onofre A. Caso Clínico: Aplicación de fibrina rica en factores de crecimiento en el tratamiento de osteonecrosis de los maxilares por Bisfosfonatos. OdontoInvestigación [Internet]. 2018 [citado 24 mayo 2025];4(2). Disponible en: https://revistas.usfq.edu.ec/index.php/odontoinvestigacion/article/view/1279
6. Park G, Benalcázar Jalkh EB, Boczar D, Bergamo E-T, Kim H, Kurgansky G, et al. Bone regeneration at extraction sockets filled with leukocyte-platelet-rich fibrin: An experimental pre-clinical study. Medicina oral, patología oral y cirugía bucal [Online]. 2022 [cited 2025 May 24];27(5): e468–e475. Available from: http://dx.doi.org/10.4317/medoral.25462
7. Valenzuela Mencía J, Gutiérrez Corrales A, Hernández A. Efectos de la fibrina rica en plaquetas y leucocitos (L-PRF) en cirugía bucal. Gaceta Dental [Internet]. 2022 [citado 24 mayo 2025]; (342):40–51. Disponible en: https://dialnet.unirioja.es/servlet/articulo?codigo=8225236
8. Murat Afat İ, Tuna Akdoğan E, Gönül O. Effects of leukocyte- and platelet-rich fibrin alone and combined with hyaluronic acid on pain, edema, and trismus after surgical extraction of impacted mandibular third molars. Journal of oral and maxillofacial surgery [Online]. 2018 [cited 2025 May 24]; 76(5):926–932. Available from: https://pubmed.ncbi.nlm.nih.gov/29304325/
9. Castro Rodrigues CMD, Sol I, De Almeida VLL, Jordao Silva C. Application of L-PRF in the field of dental surgery: A brief review. International Journal of Medical Reviews [Online]. 2021 [cited 2025 May 24];8(2):70–73. Available from: http://www.ijmedrev.com/article_133305.html
10. Serafini G, Lollobrigida M, Fortunato L, Mazzucchi G, Lamazza L, Di Nardo D, et al. Postextractive alveolar ridge preservation using L-PRF: Clinical and histological evaluation. Case reports in dentistry [Online]. 2020 [cited 2025 May 24]; 2020:5073519. Available from: https://pubmed.ncbi.nlm.nih.gov/32577315/
11. Fernandes Pacheco R, Neto Custódio AL, Souza DM de, Oliveira Pacheco CL de, Albergaria-Barbosa JR de. Concentrados autólogos de plaquetas y su aplicabilidad en Odontología. Research, Society and Development [Internet]. 2022 [citado 24 may 2025]; 11(15):e501111536838. Available from: https://rsdjournal.org/index.php/rsd/article/download/36838/31231/412612
12. Vallejo Mera D, Aguilera Molina N, Vallejo Mera V, Villacreses Rodríguez A. Efecto de la fibrina rica en plaquetas leucocitarias como biomaterial post-exodoncia para la regeneración tisular en el paciente diabético controlado. Revista Científica Especialidades Odontológicas [Internet]. 2020 [citado 24 mayo 2025]; 3(1):107–115. Available from: https://dialnet.unirioja.es/servlet/articulo?codigo=8266788
13. Canellas JVDS, da Costa RC, Breves RC, de Oliveira GP, Figueredo CM da S, Fischer RG, et al. Tomographic and histomorphometric evaluation of socket healing after tooth extraction using leukocyte- and platelet-rich fibrin: A randomized, single-blind, controlled clinical trial. Journal of cranio-maxillo-facial surgery [Online]. 2020 [cited 2025 May 24]; 48(1):24–32. Available from: https://pubmed.ncbi.nlm.nih.gov/31810848/
14. Sciaini V, Rodríguez K, Bisonni M, Nuñez M, Gomes I, Suárez JM. Utilización de fibrina rica en plaquetas y leucocitos L–PRF en defectos de lesiones periapicales y periodontales de larga evolución. RCOE: Revista del Ilustre Consejo General de Colegios de Odontólogos y Estomatólogos de España [Internet]. 2020 [citado 24 mayo 2025]; 25(2):177–185. Disponible en: https://dialnet.unirioja.es/servlet/articulo?codigo=7447507
15. Farías-Medina M, Cádiz-Marín S, Fonseca-Escobar D, Parada-Fernández F, Montero-Riffo S. Evaluación comparativa de la inflamación de tejidos blandos en pacientes sometidos a exodoncias de terceros molares con y sin uso de PRF. International Journal of Odontostomatology [Internet]. 2021 [citado 24 mayo 2025]; 15(3):674–680. Disponible en: https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300674
16. Greco T, Cabrera P, Bogarín C, Mendoza CI. Exodoncia de tercer molar con posterior utilización de plug y membrana de fibrina rica en plaquetas y leucocitos (L-PRF). Revisión Científica Odontológica UAA [Internet]. 2020 [citado 24 mayo 2025]; 2(1): 12-16. Disponible en: http://revistacientifica.uaa.edu.py/index.php/ReCO-UAA/article/download/915/831
17. Salgado-Peralvo ÁO, Salgado-García Á, Arriba-Fuente L. Nuevas tendencias en regeneración tisular: fibrina rica en plaquetas y leucocitos. Revista Española de Cirugía Oral y Maxilofacial [Internet]. 2017 [citado 24 mayo 2025]; 39(2):91–98. Disponible en: https://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-05582017000200091
18. Marchiolli CL, Lopez Moreno JM, Denardi RJ, Denardi CM, Araujo Recalde JLB, Assunção WG, et al. Platelet- and leukocyte-rich fibrin (L-PRF) in guided bone regeneration procedures. Research, Society and Development [Online]. 2021 [cited 2025 May 24]; 10(12): e130101219113. Available from: https://rsdjournal.org/index.php/rsd/article/download/19113/18257/248773
19. Araújo Júnior MA de, Castro MCSR de, Silva LSM da, Silva LS da, Corrêa CS, Nahmias HLM, et al. Aplicación terapéutica de fibrina rica en plaquetas (L-PRF) en paciente sometido a tercer exodoncia molar. Research, Society and Development [Internet]. 2022 [citado 24 mayo 2025]; 11(1):e21711124853. Disponible en: https://rsdjournal.org/index.php/rsd/article/download/24853/21808/292819
20. ElAmrousy W, Issa DR. Effect of an “autogenous leukocyte platelet-rich fibrin tooth graft” combination around immediately placed implants in periodontally compromised sites: A randomized clinical trial. International journal of dentistry [Online]. 2022 [cited 2025 May 24]; 2022:4951455. Available from: https://pubmed.ncbi.nlm.nih.gov/35242191/
21. Castro AB, Meschi N, Temmerman A, Pinto N, Lambrechts P, Teughels W, et al. Regenerative potential of leucocyte- and platelet-rich fibrin. Part B: sinus floor elevation, alveolar ridge preservation and implant therapy. A systematic review. Journal of clinical periodontology [Online]. 2017 [cited 2025 May 24]; 44(2):225–234. Available from: https://pubmed.ncbi.nlm.nih.gov/27891638/
22. Baslarli O, Tumer C, Ugur O, Vatankulu B. Evaluation of osteoblastic activity in extraction sockets treated with platelet-rich fibrin. Medicina oral, patologia oral y cirugia bucal [Online]. 2015 [cited 2025 May 24]; 20(1): e111–e116. Available from: https://pubmed.ncbi.nlm.nih.gov/25475771/
23. Lee C-H, Tsai C-Y, Li C-C, Chien C-C, Liu J-W. Teicoplanin therapy for MRSA bacteremia: a retrospective study emphasizing the importance of maintenance dosing in improving clinical outcomes. The Journal of Antimicrobial Chemotherapy [Online]. 2015 [cited 2025 May 24]; 70(1):257–263. Available from: https://pubmed.ncbi.nlm.nih.gov/25190719/
24. Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. Declaración Prisma 2020: Una guía actualizada para la publicación de revisiones sistemáticas. Revista Española de Cardiología [Internet]. 2021 [cited 2025 May 24];74(9):790–799. Disponible en: https://www.revespcardiol.org/es-declaracion-prisma-2020-una-guia-articulo-S0300893221002748
25. Da Costa CM, da Mattos Pimenta CA, Nobre MR. The PICO strategy for the research question construction and evidence search. Revista Latino-Americana de Enfermagem [Online]. 2007 [cited 2025 May 24]; 15(3): 508-511. Available from: https://pubmed.ncbi.nlm.nih.gov/17653438/
26. Higgins JPT, Green, S. Manual Cochrane de Revisiones Sistemáticas de Intervenciones. The Cochrane Collaboration. https://es.cochrane.org/sites/es.cochrane.org/files/uploads/Manual_Cochrane_510_reduit.pdf
27. Boora P, Rathee M, Bhoria M. Effect of Platelet Rich Fibrin (PRF) on peri-implant soft tissue and crestal bone in one-stage implant placement: a randomized controlled trial. Journal of Clinical and Diagnostic Research [Online]. 2015 [cited 2025 May 24];9(4): ZC18–ZC21. Available from: https://pubmed.ncbi.nlm.nih.gov/26023636/
28. Chappuis V, Engel O, Reyes M, Shahim K, Nolte L-P, Buser D. Ridge alterations post-extraction in the esthetic zone: a 3D analysis with CBCT. Journal of Dental Research [Online]. 2013 [cited 2025 May 24]; 92(12 Suppl):195S–201S. Available from: https://pubmed.ncbi.nlm.nih.gov/24158340/
29. Dohan Ehrenfest DM, Andia I, Zumstein MA, Zhang C-Q, Pinto NR, Bielecki T. Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications, and perspectives. Muscles, Ligaments and Tendons Journal [Online]. 2014 [cited 2025 May 24]; 4(1):3–9. Available from: https://pubmed.ncbi.nlm.nih.gov/24932440/
30. Farmer M, Darby I. Ridge dimensional changes following single-tooth extraction in the aesthetic zone. Clinical oral implants research [Online]. 2014 [cited 2025 May 24]; 25(2): 272–277. Available from: https://pubmed.ncbi.nlm.nih.gov/23346895/
31. Hamzacebi B, Oduncuoglu B, Alaaddinoglu EE. Treatment of peri-implant bone defects with platelet-rich fibrin. The International Journal of Periodontics & Restorative Dentistry [Online]. 2015 [cited 2025 May 24]; 35(3):415–422. Available from: https://pubmed.ncbi.nlm.nih.gov/25909530/
32. Strauss FJ, Stähli A, Gruber R. The use of platelet-rich fibrin to enhance the outcomes of implant therapy: a systematic review. Clinical Oral Implants Research [Online]. 2018 [cited 2025 May 24]; 29 Suppl 18(Suppl 18):6–19. Available from: https://pubmed.ncbi.nlm.nih.gov/30306698/
33. Nizam N, Eren G, Akcalı A, Donos N. Maxillary sinus augmentation with leukocyte and platelet-rich fibrin and deproteinized bovine bone mineral: a split-mouth histological and histomorphometric study. Clinical Oral Implants Research [Online]. 2018 [cited 2025 May 24]; 29(1):67–75. Available from: https://pubmed.ncbi.nlm.nih.gov/28786494/
34. Kanayama T, Horii K, Senga Y, Shibuya Y. Crestal approach to sinus floor elevation for atrophic maxilla using platelet-rich fibrin as the only grafting material: a 1-year prospective study. Implant Dentistry [Online]. 2016 [cited 2025 May 24]; 25(1): 32–38. Available from: https://pubmed.ncbi.nlm.nih.gov/26384097/
35. Khorshidi H, Raoofi S, Bagheri R, Banihashemi H. Comparison of the mechanical properties of early leukocyte- and platelet-rich fibrin versus PRGF/Endoret membranes. International Journal of Dentistry [Online]. 2016 [cited 2025 May 24]; 2016: 1849207. Available from: https://pubmed.ncbi.nlm.nih.gov/26880919/
36. Marenzi G, Riccitiello F, Tia M, di Lauro A, Sammartino G. Influence of leukocyte- and platelet-rich fibrin (L-PRF) in the healing of simple postextraction sockets: a split-mouth study. Biomed Research International [Online]. 2015 [cited 2025 May 24]; 2015:369273. Available from: https://pubmed.ncbi.nlm.nih.gov/26273612/
37. Öncü E, Alaaddinoğlu EE. The effect of platelet-rich fibrin on implant stability. The International Journal of Oral & Maxillofacial Implants [Online]. 2015 [cited 2025 May 24]; 30(3):578–582. Available from: https://pubmed.ncbi.nlm.nih.gov/26009908/
38. Girish Rao S, Bhat P, Nagesh KS, Rao GHR, Mirle B, Kharbhari L, et al. Bone regeneration in extraction sockets with autologous platelet rich fibrin gel. Journal of Maxillofacial and Oral Surgery [Online]. 2013 [cited 2025 May 24];12(1):11–16. Available from: https://pubmed.ncbi.nlm.nih.gov/24431807/
39. Singh A, Kohli M, Gupta N. Platelet rich fibrin: a novel approach for osseous regeneration. Journal of Maxillofacial and Oral Surgery [Online]. 2012 [cited 2025 May 24]; 11(4):430–434. Available from: https://pubmed.ncbi.nlm.nih.gov/24293936/
40. Hauser F, Gaydarov N, Badoud I, Vazquez L, Bernard J-P, Ammann P. Clinical and histological evaluation of postextraction platelet-rich fibrin socket filling: a prospective randomized controlled study. Implant Dentistry [Online]. 2013 [cited 2025 May 24];22(3):295–303. Available from: https://pubmed.ncbi.nlm.nih.gov/23644909/
41. Lollobrigida M, Maritato M, Bozzuto G, Formisano G, Molinari A, De Biase A. Biomimetic implant surface functionalization with liquid L-PRF products: in vitro study. BioMed Research International [Online]. 2018 [cited 2025 May 24]; 2018:9031435. Available from: https://pubmed.ncbi.nlm.nih.gov/29854805/
42. Kumar N, Prasad K, Ramanujam L, Ranganath K, Dexith J, Chauhan A. Evaluation of treatment outcome after impacted mandibular third molar surgery with the use of autologous platelet-rich fibrin: a randomized controlled clinical study. Journal of Oral and Maxillofacial Surgery [Online]. 2015 [cited 2025 May 24];73(6):1042–1049. Available from: https://pubmed.ncbi.nlm.nih.gov/25659357/
43. Sameera S, Nagasri M, Aravind Kumar P, Indeevar P, Raviraj K, Musalaiah SVVS. Comparison of two surgical techniques in the treatment of multiple gingival recessions sandwiched with a combination of A-PRF and L-PRF. The Saudi Dental Journal [Online]. 2018 [cited 2025 May 24]; 30(3):183–189. Available from: https://pubmed.ncbi.nlm.nih.gov/29942101/